{"id":2744,"date":"2022-05-26T15:50:27","date_gmt":"2022-05-26T14:50:27","guid":{"rendered":"https:\/\/bibliography.nanobiotix.com\/?p=2744"},"modified":"2022-05-26T16:15:40","modified_gmt":"2022-05-26T15:15:40","slug":"2020-nbtxr3-nanoparticle-with-immunoradiation-improves-survival-and-generates-long-term-anti-tumor-memory-in-an-anti-pd1-resistant-murine-lung-cancer-model","status":"publish","type":"post","link":"https:\/\/bibliography.nanobiotix.com\/fr\/2020-nbtxr3-nanoparticle-with-immunoradiation-improves-survival-and-generates-long-term-anti-tumor-memory-in-an-anti-pd1-resistant-murine-lung-cancer-model\/","title":{"rendered":"2020 \u2013 NBTXR3 nanoparticle with immunoradiation improves survival and generates long-term anti-tumor memory in an anti-PD1 resistant murine lung cancer model"},"content":{"rendered":"

[vc_row padding_top=\u00a0\u00bb0″ padding_bottom=\u00a0\u00bb0″ section_container_layout=\u00a0\u00bbfull-width\u00a0\u00bb remove_horizontal_padding=\u00a0\u00bbyes\u00a0\u00bb module_type=\u00a0\u00bbbg-color\u00a0\u00bb gutter_size=\u00a0\u00bbyes\u00a0\u00bb equal_height=\u00a0\u00bbyes\u00a0\u00bb bg_color=\u00a0\u00bb#28282e\u00a0\u00bb][vc_column][vc_row_inner padding_top=\u00a0\u00bb0″ padding_bottom=\u00a0\u00bb0″ gutter_size=\u00a0\u00bbyes\u00a0\u00bb equal_height=\u00a0\u00bbyes\u00a0\u00bb][vc_column_inner column_paddings=\u00a0\u00bb105″ column_position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb column_min_height=\u00a0\u00bb700″ column_min_height_sm=\u00a0\u00bb400″ column_min_height_xs=\u00a0\u00bb350″ module_type=\u00a0\u00bbbg-image\u00a0\u00bb bg_image=\u00a0\u00bb99″ mask_fx=\u00a0\u00bbyes-mask\u00a0\u00bb mask_color_mode=\u00a0\u00bbpalette\u00a0\u00bb mask_bg_color_palette=\u00a0\u00bbmain-mask\u00a0\u00bb animation_fx=\u00a0\u00bbyes-animation\u00a0\u00bb animation_delay=\u00a0\u00bb200″ animation_offset_scroll_down=\u00a0\u00bb90″ width=\u00a0\u00bb1\/2″ animation_in=\u00a0\u00bbfadeInUp\u00a0\u00bb][vc_empty_space height=\u00a0\u00bb60px\u00a0\u00bb responsive_lg=\u00a0\u00bbhidden\u00a0\u00bb responsive_md=\u00a0\u00bbhidden\u00a0\u00bb][az_box_icons box_icon_title=\u00a0\u00bbAuthors\u00a0\u00bb box_icon_color_mode=\u00a0\u00bbon-the-fly\u00a0\u00bb icon_visibility=\u00a0\u00bbyes-icon\u00a0\u00bb icon_type=\u00a0\u00bbfont\u00a0\u00bb icon=\u00a0\u00bbfa fa-edit\u00a0\u00bb icon_color_mode=\u00a0\u00bbon-the-fly\u00a0\u00bb icon_size=\u00a0\u00bb50″ box_icon_color=\u00a0\u00bb#ffffff\u00a0\u00bb icon_color=\u00a0\u00bb#ffffff\u00a0\u00bb]Yun Hu1<\/span>, James Welsh2<\/span>, Sebastien Paris1<\/span>, Hampartsoum Barsoumian1<\/span>, Chike Abana1<\/span>, Saumil Gandhi1<\/span>, Quynh Nguyen1<\/span>, Maria Cortez
\n
\n1 \u2014 MD Anderson Cancer Center, Houston, TX, USA
\n2 \u2014 Nanobiotix, Paris, France
\n<\/span>[\/az_box_icons][vc_empty_space height=\u00a0\u00bb60px\u00a0\u00bb responsive_lg=\u00a0\u00bbhidden\u00a0\u00bb responsive_md=\u00a0\u00bbhidden\u00a0\u00bb][\/vc_column_inner][vc_column_inner column_paddings=\u00a0\u00bb105″ column_position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb module_type=\u00a0\u00bbbg-color\u00a0\u00bb animation_fx=\u00a0\u00bbyes-animation\u00a0\u00bb animation_delay=\u00a0\u00bb300″ animation_offset_scroll_down=\u00a0\u00bb90″ width=\u00a0\u00bb1\/2″ bg_color=\u00a0\u00bb#ffffff\u00a0\u00bb animation_in=\u00a0\u00bbfadeInUp\u00a0\u00bb][vc_empty_space height=\u00a0\u00bb60px\u00a0\u00bb responsive_lg=\u00a0\u00bbhidden\u00a0\u00bb responsive_md=\u00a0\u00bbhidden\u00a0\u00bb][az_box_icons box_icon_title=\u00a0\u00bbSummary\u00a0\u00bb icon_visibility=\u00a0\u00bbyes-icon\u00a0\u00bb icon_type=\u00a0\u00bbfont\u00a0\u00bb icon=\u00a0\u00bbaz-icon az-icon-layers2″ icon_color_mode=\u00a0\u00bbon-the-fly\u00a0\u00bb icon_color=\u00a0\u00bb#28282e\u00a0\u00bb icon_size=\u00a0\u00bb50″]Background:<\/b> Although treatment with high-dose (HD) radiation (XRT) and NBTXR3 on primary tumors in combination with systemic anti-PD1 was able to significantly improve abscopal effect in 344SQR murine metastatic lung cancer model, most of the mice eventually died due to the growth of secondary tumors. Therefore, we intended to use HD-XRT plus NBTXR3 injection into primary tumors and low-dose (LD) radiation on secondary tumors plus dual-agent immunotherapy (IT) of anti-PD1 and anti-CTLA-4 to acheive complete control of both the primary and secondary tumors in mice.<\/p>\n

Methods:<\/b> Five groups of 8 mice each were inoculated subcutaneously with 5 \u00d7 104 anti-PD1-resistant 344SQR cells in each hind leg, 3 days apart, to establish \u2018primary\u2019 (right) and \u2018secondary\u2019 (left) tumors. All mice in treatment groups received intraperitoneal anti-PD1 and anti-CTLA-4 on days 4, 7, 10, and 13, and continuing anti-PD1 treatment on days 20, 27, 34, 41, and 49 and 12Gy x3 (HD-XRT) to the primary tumors on days 7, 8 and 9. Primary tumors in groups 3 and 5 also received intratumoral NBTXR3 on day 6. Secondary tumors in groups 4 and 5 were also irradiated with 1Gyx2 (LD-XRT) on days 12 and 13. Experimental groups were designated as 1=Control, 2=HD+IT, 3=NBTXR3+HD+IT, 4=HD+LD+IT, and 5=NBTXR3+HD+LD+IT. The secondary tumors were analyzed by flow cytometry and Nanostring. On day 178, the survivor mice were rechallenged with 5 \u00d7 104 344SQR cells on the right flank and the tumor growth was monitored for an additional 36 days.<\/p>\n

Results:<\/b> All mice in all the groups except NBTXR3+HD+LD+IT died due to the growth of either the primary tumor or the secondary tumor by day 36. Both the primary and the secondary tumors in 4 mice of NBTXR3+HD+LD+IT group were completely eliminated. No tumor growth was observed in these mice after rechallenged with 344SQR cells. Flow cytometry data demonstrated that only the mice in the groups with NBTXR3 had significantly more CD8+ T cell infiltration in the secondary tumor collected on day 16 than the control. Both flow cytometry and Nanostring data showed that only the mice in NBTXR3+HD+LD+IT had a significantly higher CD8+ Tcell\/Treg cell ratio than the control.<\/p>\n

Conclusions:<\/b> The combination of NBTXR3 plus high and low dose radiation with immunotherapy effectively controlled the growth of both primary and secondary tumors, significantly extended the survival, generating long-term antitumor memory. This combination therapy induced immune-mediated control of the secondary tumor at both genetic and cellular levels.[\/az_box_icons][vc_empty_space height=\u00a0\u00bb60px\u00a0\u00bb responsive_lg=\u00a0\u00bbhidden\u00a0\u00bb responsive_md=\u00a0\u00bbhidden\u00a0\u00bb][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row]<\/p>\n<\/section>","protected":false},"excerpt":{"rendered":"

Although treatment with high-dose (HD) radiation (XRT) and NBTXR3 on primary tumors in combination with systemic anti-PD1 was able to significantly improve abscopal effect in 344SQR murine metastatic lung cancer model, most of the mice eventually died due to the growth of secondary tumors. [\u2026]<\/p>\n","protected":false},"author":1,"featured_media":2795,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[182,184,666],"tags":[539,686,687,342,203],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/bibliography.nanobiotix.com\/fr\/wp-json\/wp\/v2\/posts\/2744"}],"collection":[{"href":"https:\/\/bibliography.nanobiotix.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bibliography.nanobiotix.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bibliography.nanobiotix.com\/fr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/bibliography.nanobiotix.com\/fr\/wp-json\/wp\/v2\/comments?post=2744"}],"version-history":[{"count":1,"href":"https:\/\/bibliography.nanobiotix.com\/fr\/wp-json\/wp\/v2\/posts\/2744\/revisions"}],"predecessor-version":[{"id":2796,"href":"https:\/\/bibliography.nanobiotix.com\/fr\/wp-json\/wp\/v2\/posts\/2744\/revisions\/2796"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bibliography.nanobiotix.com\/fr\/wp-json\/wp\/v2\/media\/2795"}],"wp:attachment":[{"href":"https:\/\/bibliography.nanobiotix.com\/fr\/wp-json\/wp\/v2\/media?parent=2744"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bibliography.nanobiotix.com\/fr\/wp-json\/wp\/v2\/categories?post=2744"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bibliography.nanobiotix.com\/fr\/wp-json\/wp\/v2\/tags?post=2744"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}