Nanosized | Nano Publications https://bibliography.nanobiotix.com Navigate through all Nanobiotix publications online Tue, 24 May 2022 15:27:22 +0000 en-US hourly 1 https://wordpress.org/?v=6.0 https://bibliography.nanobiotix.com/wp-content/uploads/2019/11/cropped-n-icon-512x512-32x32.png Nanosized | Nano Publications https://bibliography.nanobiotix.com 32 32 2017 – Abstract SITC Conference Maryland – Clinical https://bibliography.nanobiotix.com/2017-abstract-sitc-conference-maryland-clinical/ https://bibliography.nanobiotix.com/2017-abstract-sitc-conference-maryland-clinical/#respond Tue, 07 Nov 2017 06:54:45 +0000 http://bibliography.nanobiotix.com/?p=1232 Soft tissue sarcoma (STS) is a large and heterogeneous group of malignant mesenchymal neoplasms characterized by a strong tendency toward local recurrence and metastatic spreading. Consistently, the immune microenvironment in sarcomas is highly variable. A new class of material with high electron density, hafnium oxide, was designed at the nanoscale to efficiently absorb ionizing radiation […]

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Authors

Galon J.1, Laé M.2, Thariat J.3, Carrere S.4, Papai Z.5, Delannes M.6, Rochaix P.6, Mangel L.7, Hermitte F.8, Sapi Z.9, Tornoczky T.7, Servois V.2, Birtwisle Peyrottes I.3, Tetreau R.4, Château M-C.4, Paris S.10, Brisse H.2, Bonvalot S.2
1 – INSERM, Paris, France
2 – Institut Curie, Paris, France
3 – Centre Antoine Lacassagne, Nice, France
4 – Centre regional de lutte contre le cancer, Paul Lamarque, Montpellier
5 – Medical Centre Hungarian Defences forces, Budapest, Hungary
6 – Institut Universitaire du Cancer Toulouse-Oncopole, Toulouse, France
7 – Pecs University, Pecs, Hungary
8 – HalioDx, Marseille, France
9 – Semmelweis University, Budapest, Hungary
10 – Nanobiotix, Paris, France

Summary

Background: Soft tissue sarcoma (STS) is a large and heterogeneous group of malignant mesenchymal neoplasms characterized by a strong tendency toward local recurrence and metastatic spreading. Consistently, the immune microenvironment in sarcomas is highly variable. A new class of material with high electron density, hafnium oxide, was designed at the nanoscale to efficiently absorb ionizing radiation from within the tumor cells and augment the dose deposited to a tumor. […]

Material and Methods: Tumor tissues pre- (biopsy) and post-treatment (resection) are collected from patients with locally advanced STS (NCT02379845), who received either HfO2-NP activated by RT or RT alone. Immunohistochemistry and Digital Pathology for immune biomarkers and Pan-Immune gene expression profiling are analyzed.

Results: A significant increase of CD8+ T cells and a marked increase of CD3+ and PD-1 T cells and CD103+ immune cell infiltration post- vs pre-treatment are observed for HfO2-NP + RT while not differences are seen for RT alone (more than 10 patients analyzed in each arm). Functional analysis of genes expression up-regulated in HfO2-NP + RT post- vs pre-treatment shows an enrichment of cytokine activity (IL7, IFNA, IL11, IFNG), adaptive immunity (RAG1, TAP1, TAP2, TBX21, IFNG, LTK, CD37, CD22) and T cell receptor signaling pathway (CD28, CTLA4, CD274, BTLA, TIGIT, CD5, ZAP70) when compared to RT.

Conclusions: Promising results are observed in patients who received HfO2-NP + RT in terms of immune cells infiltration post- vs pre-treatment when compared to RT. […]

The post 2017 – Abstract SITC Conference Maryland – Clinical first appeared on Nano Publications.]]> https://bibliography.nanobiotix.com/2017-abstract-sitc-conference-maryland-clinical/feed/ 0 2017 – Abstract SITC Conference Maryland – Non Clinical https://bibliography.nanobiotix.com/2017-abstract-sitc-conference-maryland-non-clinical/ https://bibliography.nanobiotix.com/2017-abstract-sitc-conference-maryland-non-clinical/#respond Tue, 07 Nov 2017 07:50:03 +0000 http://bibliography.nanobiotix.com/?p=1252 Hafnium oxide, an electron-dense material, was designed at the nanoscale to increase the radiation dose deposited from within the cancer cells: “Hot spot” of energy deposit where the nanoparticles are when exposed to radiation therapy (RT). Preclinical studies have demonstrated increase of cancer cells killing in vitro and marked antitumor efficacy in vivo with presence of these nanoparticles […]

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Authors

Sébastien Paris, Audrey Darmon, Ping Zhang, Maxime Bergère, Laurent Levy
Nanobiotix, 60 rue de wattignies, 75012 Paris, France

Summary

Background: Hafnium oxide, an electron-dense material, was designed at the nanoscale to increase the radiation dose deposited from within the cancer cells: “Hot spot” of energy deposit where the nanoparticles are when exposed to radiation therapy (RT). Preclinical studies have demonstrated increase of cancer cells killing in vitro and marked antitumor efficacy in vivo with presence of these nanoparticles (HfO2-NP) exposed to RT, when compared to RT alone. […]

Material and Methods: CT26 (murine colorectal cancer cells) were subcutaneously injected in the flank of BALB/c mice. Once the mean tumors volume reached 115±30 mm3, tumors were intratumor injected with HfO2-NP and irradiated with 2Gyx3 or 4Gyx3, or irradiated only. Tumors were collected 5 days after the last RT fraction and analyzed for immune cell infiltrates by immunohistochemistry (2Gyx3 and 4Gyx3) and cytokines content by flow cytometry (2Gyx3). […]

Results: In mice bearing CT26 tumors, a marked increase of cytokines content and immune cell infiltrates was observed with HfO2-NP + 2Gyx3 when compared to RT alone. The tumor immune cell infiltrates were
further enhanced with HfO2-NP + 4Gyx3. In mice inoculated with 4T1 cells treated with HfO2-NP + 40Gy, a marked increase of immune cell infiltrate (CD8+) was observed in tumors when compared to tumors in mice inoculated with 4T1 cells treated with 40Gy and control.

Conclusions: These in vivo data generated from CT26 and 4T1 tumor models suggest that HfO2-NP + RT triggers immunogenic conversion of the tumor microenvironement when compared to RT alone. HfO2-NP treatment may represent a therapeutical approach for broad applications since it does not rely on any molecular characteristics of the tumor.

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